![]() ![]() Primer3 is free and offered on an "as-is", and use-at-your-own-risk basis. The program can be run on Windows, Linux/Unix, and other systems. It is also able to select primers for a single sequence using a web-based interface. It can design primers for multiple sequences using batch mode command line style. Primer3: Primer3 is a free program commonly used for designing PCR primers. Clustal is available for a number of platforms including, Windows, Linux, FreeDSD and Mac. Omega is the newest version and provides an increase in scalability, so that hundreds of thousands of sequences can be aligned quickly (within a few hours). It provides an integrated environment for performing multiple sequence and profile alignments and analyzing alignment results. It offers search, database and filtering applications.Ĭlustal: The Clustal multiple alignment of nucleic acid and protein sequences is available in command-line or graphical interface and can be installed on your computer or run online. Instructions and options can be found on the NCBI website. ![]() BLAST+: This is a standalone version of BLAST that can be installed in your PC and run in MS-DOS mode as well as platforms, including general Linux and Unix systems.
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